1D43

DNA DODECAMER C-G-C-G-A-A-T-T-C-G-C-G/HOECHST 33258 COMPLEX: 0 DEGREES C, PIPERAZINE UP

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	273
Detector technology	DIFFRACTOMETER
Detector	RIGAKU AFC-5R
Spacegroup name	P 21 21 21
Unit cell lengths	23.950, 38.990, 65.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.157
Refinement software	NUCLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.178 *
Number of reflections	2540 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	4 *	pH 6.90, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER
2	1	1	MPD
3	1	1	MG ACETATE
4	1	1	SPERMINE_HCL
5	1	2	WATER
6	1	2	MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DNA	0.22 (mM)
2	1	drop	Hoechest 33258	0.44 (mM)
3	1	drop		5.0 (mM)
4	1	drop	spermine-HCl	0.5 (mM)
5	1	drop	MPD	15 (%(v/v))
6	1	reservoir		32.5 (%(v/v))