1D3S

1.4 A crystal structure of nitrophorin 4 from Rhodnius prolixis at pH=5.6.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	140
Detector technology	DIFFRACTOMETER
Collection date	1999-05-06
Detector	ENRAF-NONIUS FAST
Spacegroup name	C 1 2 1
Unit cell lengths	70.180, 42.520, 52.860
Unit cell angles	90.00, 94.35, 90.00

Refinement procedure

Resolution	30.000 - 1.400
R-factor	0.2 *
Rwork	0.210
R-free	0.24000 *
RMSD bond length	0.010
RMSD bond angle	0.030
Data reduction software	MADNESS
Data scaling software	SCALA
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.120	0.150
Total number of observations	66833 *
Number of reflections	25227
<I/σ(I)>	10.3
Completeness [%]	80.0	79
Redundancy	2.65	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	23% PEG 4000 20 MM SODIUM CITRATE PH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	PEG4000	25 (%(w/v))
4	1	reservoir	sodium citrate	100 (mM)