1D3K

HUMAN SERUM TRANSFERRIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	45.010, 57.810, 135.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.800
Rwork	0.184
R-free	0.22500
RMSD bond length	0.008
RMSD bond angle	1.410
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.069	0.284
Number of reflections	30624
<I/σ(I)>	15.3
Completeness [%]	91.0	96
Redundancy	2.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.75	4 *	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	sodium cacodylate	40 (mM)
3	1	reservoir	PEG4000	26 (%)
4	1	reservoir	sodium bicarbonate	20 (mM)