1D39
COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Detector | SYNTEX P2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 18.010, 31.030, 44.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.200 |
| R-factor | 0.198 |
| Starting model (for MR) | ZDF001 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.200 |
| Number of reflections | 4107 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | referred to 2dcg * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 2 | WATER | ||
| 5 | 1 | 2 | ISOPROPANOL | ||
| 6 | 1 | 3 | WATER | ||
| 7 | 1 | 3 | CUCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium cacodylate | 30 (mM) | |
| 2 | 1 | reservoir | spermine tetrachloride | 10 (mM) | |
| 3 | 1 | reservoir | 15 (mM) | ||
| 4 | 1 | reservoir | d(CG)3 | 2 (mM) |
