1D30
THE STRUCTURE OF DAPI BOUND TO DNA
Experimental procedure
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.350, 40.710, 66.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.222 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.116 * |
| Number of reflections | 2541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 4 * | pH 7.00, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MG ACETATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 0.28 (mM) | |
| 2 | 1 | drop | spermine-HCl | 0.22 (mM) | |
| 3 | 1 | drop | magnesium acetate | 5.38 (mM) | |
| 4 | 1 | drop | 4'-6-diamidine-2-phenyl indole | 0.23 (mM) | |
| 5 | 1 | drop | MPD | 20 (%(v/v)) | |
| 6 | 1 | reservoir | 40 (%(v/v)) |
