1D2T

CRYSTAL STRUCTURE OF ACID PHOSPHATASE FROM ESCHERICHIA BLATTAE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-22
Detector	FUJI
Spacegroup name	P 63 2 2
Unit cell lengths	124.700, 124.700, 97.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.215
Rwork	0.216
R-free	0.25700
RMSD bond length	0.017 *
RMSD bond angle	0.017
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.074 *	0.341
Number of reflections	34749 *
<I/σ(I)>	5.72
Completeness [%]	92.7	74.4
Redundancy	4.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	20 *	drop contains protein and reservoir solution in a 1:1 ratio *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	reservoir	PEG400	43-45 (%)
4	1	reservoir	Tris-HCl	0.1 (M)