1D2S
CRYSTAL STRUCTURE OF THE N-TERMINAL LAMININ G-LIKE DOMAIN OF SHBG IN COMPLEX WITH DIHYDROTESTOSTERONE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 104.040, 104.040, 84.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.550 |
| R-factor | 0.21 |
| Rwork | 0.205 |
| R-free | 0.25100 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.750 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.042 | 0.234 |
| Number of reflections | 24678 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 96.4 | 95 |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | 2.5 (mM) | ||
| 4 | 1 | drop | 5alpha-DHT | 0.003 (mM) | |
| 5 | 1 | reservoir | isopropanol | 20 (%) | |
| 6 | 1 | reservoir | PEG400 | 10 (%) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) |
