1D2R

2.9 A CRYSTAL STRUCTURE OF LIGAND-FREE TRYPTOPHANYL-TRNA SYNTHETASE: DOMAIN MOVEMENTS FRAGMENT THE ADENINE NUCLEOTIDE BINDING SITE.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Spacegroup name	C 1 2 1
Unit cell lengths	226.760, 91.660, 156.970
Unit cell angles	90.00, 132.66, 90.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.25 *
Rwork	0.237
R-free	0.28000 *
RMSD bond length	0.009
RMSD bond angle	1.140 *
Data reduction software	DENZO
Phasing software	RSREF
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000
High resolution limit [Å]	2.900
Rmerge	0.091
Number of reflections	38244
Completeness [%]	75.0 *	55 *
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.8	315	2.0 M K2HPO4 , pH 6.8, MICRODIALYSIS, temperature 315K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	potassium phosphate	2.0 (M)