1D2A
CRYSTAL STRUCTURE OF A YEAST LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE (LTP1) COMPLEXED WITH THE ACTIVATOR ADENINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-06 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.492, 63.272, 97.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.24400 * |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.420 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.149 |
| Total number of observations | 327192 * | |
| Number of reflections | 22621 | |
| <I/σ(I)> | 35.1 | |
| Completeness [%] | 87.6 | 39.3 |
| Redundancy | 14.5 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 20 * | PEG 3400, Bis-TRIS, sodium chloride, sodium phosphate, adenine, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG3400 | 12-18 (%) | |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | bis-tris | 100 (mM) | |
| 5 | 1 | reservoir | sodium phosphate | 20 (mM) | |
| 6 | 1 | reservoir | adenine | 20 (mM) |
