1D27
HIGH-RESOLUTION STRUCTURE OF A MUTAGENIC LESION IN DNA
Experimental procedure
| Source type | ROTATING ANODE |
| Detector technology | DIFFRACTOMETER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.440, 40.700, 65.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.000 |
| R-factor | 0.185 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.029 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.030 * |
| Number of reflections | 4557 |
| Completeness [%] | 91.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 277 | pH 6.30, VAPOR DIFFUSION, temperature 277.00K |
| 1 | VAPOR DIFFUSION | 6.3 | 277 | pH 6.30, VAPOR DIFFUSION, temperature 277.00K |
| 1 | VAPOR DIFFUSION | 6.3 | 277 | pH 6.30, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | HEXYLENE GLYCOL | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | NA CACODYLATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 1.0 (mM) | |
| 2 | 1 | drop | 33 (mM) | ||
| 3 | 1 | drop | hexylene glycol | 16.7 (%(v/v)) | |
| 4 | 1 | drop | spermine | 1.25 (mM) | |
| 5 | 1 | drop | sodium cacodylate | 5 (mM) |
