1D1U
USE OF AN N-TERMINAL FRAGMENT FROM MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE TO FACILITATE CRYSTALLIZATION AND ANALYSIS OF A PSEUDO-16-MER DNA MOLECULE CONTAINING G-A MISPAIRS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 108 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.738, 145.491, 46.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.28600 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.069 | 0.278 |
| Number of reflections | 17117 | |
| <I/σ(I)> | 22.8 | |
| Completeness [%] | 98.9 | 98.5 |
| Redundancy | 5.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 293 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.45 (mM) | |
| 2 | 1 | drop | oligonucleotide | 0.9 (mM) | |
| 3 | 1 | drop | ddCTP | 3.6 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 10 (%) | |
| 5 | 1 | reservoir | 0.1 (M) | ||
| 6 | 1 | reservoir | ADA | 0.05 (M) |
