1D1L

CRYSTAL STRUCTURE OF CRO-F58W MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-01-03
Detector	FUJI
Spacegroup name	F 2 2 2
Unit cell lengths	59.860, 67.300, 91.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.181
R-free	0.25800
RMSD bond length	0.014
RMSD bond angle	2.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.100
Rmerge	0.044
Total number of observations	24029 *
Number of reflections	5304
Completeness [%]	93.0
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	2.2 M NH4SO4, 6% isopropanol, including a layer of Hampton's "Al's oil", pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.2 (M)
2	1	reservoir	isopropanol	6 (%(w/v))
3	1	drop	protein	11 (mg/ml)
4	1	drop	Al's oil