1D1K
MUTATED SHIGA-LIKE TOXIN B SUBUNIT (D17E/W34A) COMPLEXED WITH RECEPTOR GB3 ANALOGUE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-02-12 |
| Detector | SIEMENS |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.223, 44.131, 53.710 |
| Unit cell angles | 106.05, 106.38, 99.26 |
Refinement procedure
| Resolution | 25.800 - 2.000 |
| Rwork | 0.193 |
| R-free | 0.21800 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.800 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.330 |
| Number of reflections | 22215 | |
| <I/σ(I)> | 12.17 | |
| Completeness [%] | 91.0 | 41.1 |
| Redundancy | 1.73 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.4 M K,NA TARTRATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
