1D16
STRUCTURE OF A T4 HAIRPIN LOOP ON A Z-DNA STEM AND COMPARISON WITH A-RNA AND B-DNA LOOPS
Experimental procedure
| Temperature [K] | 278 |
| Detector technology | DIFFRACTOMETER |
| Detector | SYNTEX P1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.180, 21.630, 36.400 |
| Unit cell angles | 90.00, 95.22, 90.00 |
Refinement procedure
| Resolution | ? - 2.100 |
| R-factor | 0.2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.043 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 2825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 7 * | pH 6.00, VAPOR DIFFUSION, temperature 280.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | ISOPROPANOL | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 1 | SR NITRATE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | ISOPROPANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 2 | 1 | drop | spermine tetrachloride | 20 (mM) | |
| 3 | 1 | drop | strontium nitrate | 5 (mM) | |
| 4 | 1 | drop | DNA | 1 (mM) | |
| 5 | 1 | reservoir | isopropanol | 40 (%) |
