1D15
TERNARY INTERACTIONS OF SPERMINE WITH DNA: 4'-EPIADRIAMYCIN AND OTHER DNA: ANTHRACYCLINE COMPLEXES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 28.040, 28.040, 53.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.196 |
| RMSD bond length | 0.019 |
| Refinement software | PROLSQ ((MODIFIED BY G.J.QUIGLEY)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 1714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | pH 6.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1.2 (mM) | single strand |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | spermine | 2.5 (mM) | |
| 4 | 1 | drop | 1.2 (mM) | ||
| 5 | 1 | drop | MPD | 8 (%) | |
| 6 | 1 | reservoir | MPD | 30 (%) |
