1D0Q

STRUCTURE OF THE ZINC-BINDING DOMAIN OF BACILLUS STEAROTHERMOPHILUS DNA PRIMASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-08-08
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	36.170, 58.745, 45.750
Unit cell angles	90.00, 91.73, 90.00

Refinement procedure

Resolution	15.000 - 1.710
R-factor	0.196 *
Rwork	0.204
R-free	0.22500
RMSD bond length	0.010 *
RMSD bond angle	2.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.780
High resolution limit [Å]	1.710	1.700
Rmerge	0.047	0.227
Number of reflections	22075
<I/σ(I)>	14.95
Completeness [%]	95.9	94.7
Redundancy	1.78	1.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	used microseeding *
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	drop	EDTA	2 (mM)
4	1	reservoir		60 (%sat)
5	1	reservoir	zinc acetate	1 (mM)
6	1	reservoir	sodium citrate	100 (mM)