1D0L

THE ESCHERICHIA COLI LYTIC TRANSGLYCOSYLASE SLT35 IN COMPLEX WITH BULGECIN A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1998-11-29
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	58.395, 67.785, 97.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.970
R-factor	0.17
Rwork	0.168
R-free	0.20000
RMSD bond length	0.006
RMSD bond angle	21.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.800	2.000
High resolution limit [Å]	1.970	1.970
Rmerge	0.053	0.249
Total number of observations	205843 *
Number of reflections	27987
<I/σ(I)>	33.4
Completeness [%]	99.3	85
Redundancy	7.3	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	295	BICINE-NAOH, ISOPRAPANOL, PEG20K, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	bicine-NaOH	0.1 (M)