1D0K

THE ESCHERICHIA COLI LYTIC TRANSGLYCOSYLASE SLT35 IN COMPLEX WITH TWO MURODIPEPTIDES (GLCNAC-MURNAC-L-ALA-D-GLU)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1997-10-29
Detector	MACSCIENCE
Spacegroup name	P 21 21 21
Unit cell lengths	58.379, 67.825, 98.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.020
R-factor	0.168
Rwork	0.165
R-free	0.20200
RMSD bond length	0.006
RMSD bond angle	21.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.200	2.050
High resolution limit [Å]	2.020	2.020
Rmerge	0.067	0.267
Total number of observations	108180 *
Number of reflections	26200
<I/σ(I)>	20.7
Completeness [%]	99.0	99.9
Redundancy	4.1	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	295	BICINE-NAOH, ISOPROPANOL, PEG20K, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	bicine-NaOH	0.1 (M)