1D0J

STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 IN COMPLEX WITH A M4-1BB PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-02-01
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	135.900, 85.600, 124.100
Unit cell angles	90.00, 119.10, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.227
Rwork	0.227
R-free	0.27000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500	2.500
Rmerge	0.061	0.296
Completeness [%]	90.2	85.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6	293	PEG4K, MES PH 6.0, VAPOR DIFFUSION, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	8-15 (%)
2	1	1	MES	0.1 (M)