1D02
CRYSTAL STRUCTURE OF MUNI RESTRICTION ENDONUCLEASE IN COMPLEX WITH COGNATE DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 77 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.400, 72.470, 121.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.700 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.21300 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.490 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | |
High resolution limit [Å] | 1.700 | |
Rmerge | 0.052 | |
Total number of observations | 177834 * | |
Number of reflections | 56945 | |
Completeness [%] | 93.6 | 84 * |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 20 * | PEG 8000, CALCIUM CHLORIDE, SODIUM CHLORIDE, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.2 (mM) | |
2 | 1 | reservoir | PEG8000 | 14 (%) | |
3 | 1 | reservoir | 200 (mM) | ||
4 | 1 | reservoir | 50 (mM) | ||
5 | 1 | reservoir | MES | 0.1 (M) |