1D01
STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 IN COMPLEX WITH A HUMAN CD30 PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-02-01 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.300, 110.400, 91.300 |
| Unit cell angles | 90.00, 120.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.24400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 | 0.199 |
| Number of reflections | 94994 | |
| Completeness [%] | 96.5 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 293 | PEG4K, MES, PH 6.0, VAPOR DIFFUSION, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 8-15 (%) | |
| 2 | 1 | 1 | MES | 0.1 (M) |
