1D00

STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 IN COMPLEX WITH A 5-RESIDUE CD40 PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	110
Detector technology	CCD
Collection date	1998-12-01
Detector	ADSC
Spacegroup name	R 3
Unit cell lengths	111.400, 111.400, 111.400
Unit cell angles	103.70, 103.70, 103.70

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.219
R-free	0.25400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000	2.000
Rmerge	0.055	0.180
Completeness [%]	100.0	99.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6	293	PEG4K, MES, Ph6.0, VAPOR DIFFUSION, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	8-15 (%)
2	1	reservoir	MES	0.1 (M)