1CZZ

STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 IN COMPLEX WITH A 17-RESIDUE CD40 PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	1998-01-12
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	59.400, 81.100, 77.200
Unit cell angles	90.00, 96.80, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.221
Rwork	0.221
R-free	0.26700
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.700 *
Rmerge	0.044 *	0.255 *
Completeness [%]	98.6 *	99.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	293	PEG4K, MES, PH 5.6, VAPOR DIFFUSION, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	11 (%)
2	1	1	ammonium acetate	0.2 (M)
3	1	1	citrate	0.1 (M)