1CZY

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN THE TRAF DOMAIN OF HUMAN TRAF2 AND AN LMP1 BINDING PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	56.200, 76.800, 66.900
Unit cell angles	90.00, 93.20, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.206
Rwork	0.206
R-free	0.24500
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.000 *
Rmerge	0.055 *	0.150 *
Completeness [%]	99.7 *	100 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6	293	PEG4K, MES BUFFER, pH 6.00, VAPOR DIFFUSION, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	8-15 (%)
2	1	1	MES	0.1 (M)