1CZT
CRYSTAL STRUCTURE OF THE C2 DOMAIN OF HUMAN COAGULATION FACTOR V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.890, 53.330, 68.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.870 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1czs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.790 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.730 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.061 | 0.287 |
| Number of reflections | 13815 | |
| Completeness [%] | 94.0 | 86.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 6 * | AMMONIUM SULFATE, pH 10.20, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MOPS | 5 (mM) | pH8.0 |
| 2 | 1 | drop | protein | 6 (mg/ml) | |
| 3 | 1 | reservoir | Tris | 0.1 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.4 (M) | pH10.2 |
