1CZK
COMPARISONS OF WILD TYPE AND MUTANT FLAVODOXINS FROM ANACYSTIS NIDULANS. STRUCTURAL DETERMINANTS OF THE REDOX POTENTIALS.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 1991-06-01 |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.170, 66.170, 51.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.157 |
| Rwork | 0.157 |
| R-free | 0.20000 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.990 | |
| High resolution limit [Å] | 1.800 | 1.900 |
| Rmerge | 0.064 | |
| Number of reflections | 17961 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 91.9 | 90 * |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 4 * | used to seeding, Drennan, C.L., (1999) J. Mol. Biol., 294, 711. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.5-10.2 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 0.96-1.4 (M) | |
| 3 | 1 | drop | potassium phosphate | 0.06-0.075 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.4-2.8 (M) | |
| 5 | 1 | reservoir | phosphate | 0.15 (mM) |
