1CZJ

CYTOCHROME C OF CLASS III (AMBLER) 26 KD

Experimental procedure

Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-02-13
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	73.710, 73.710, 57.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 2.160
R-factor	0.204
Rwork	0.204
R-free	0.26300
RMSD bond length	0.009 *
RMSD bond angle	1.790 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.800 *
High resolution limit [Å]	2.160
Rmerge	0.089
Total number of observations	28293 *
Number of reflections	9198
Completeness [%]	92.4
Redundancy	3.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8	4 *	Czjzek, M., (1992) J. Mol. Biol., 228, 995. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	0.4 (M)
3	1	reservoir	ethanol	13 (%)
4	1	reservoir	Tris-HCl	0.4 (M)