1CZE

ASPARTATE AMINOTRANSFERASE MUTANT ATB17/139S/142N WITH SUCCINIC ACID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1999-02-16
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 2 2 21
Unit cell lengths	156.640, 85.060, 78.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.195
Rwork	0.189
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yoo
RMSD bond length	0.008
RMSD bond angle	23.800 *
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.064	0.123
Total number of observations	71147 *
Number of reflections	18949
<I/σ(I)>	15.9	42.5
Completeness [%]	99.7	99.4
Redundancy	3.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	20 *	3 micro litter of protein was mixed with 1 microl litter of sodium succinate and 3 micro litter of a reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein/peptide	37 (mg/ml)
2	1	drop	sodium succinate	1 (M)
3	1	reservoir	ammonium sulfate	1.7 (M)
4	1	reservoir	HEPES	0.1 (M)