1CZD
CRYSTAL STRUCTURE OF THE PROCESSIVITY CLAMP GP45 FROM BACTERIOPHAGE T4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.177, 93.938, 141.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.450 |
| R-factor | 0.2322 * |
| Rwork | 0.232 |
| R-free | 0.25900 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.293 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.096 | 0.290 |
| Total number of observations | 282837 * | |
| Number of reflections | 30934 * | |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 92.5 | 95.4 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 4 * | 60 mM PIPES 200 mM CASO4 0.1 % 1,4 Dioxane 15% Glycerol 15% PEG MME 5000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PIPES | 60 (mM) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | 1,4-dioxane | 0.1 (%(v/v)) | |
| 4 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
| 5 | 1 | reservoir | PEG5000 MME | 15 (%(v/v)) | |
| 6 | 1 | drop | PHMB | 0.1 (mM) | |
| 7 | 1 | drop | Tris | 10 (mM) |
