1CZD

CRYSTAL STRUCTURE OF THE PROCESSIVITY CLAMP GP45 FROM BACTERIOPHAGE T4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1995-01-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	66.177, 93.938, 141.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.450
R-factor	0.2322 *
Rwork	0.232
R-free	0.25900
RMSD bond length	0.007
RMSD bond angle	1.293
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.096	0.290
Total number of observations	282837 *
Number of reflections	30934 *
<I/σ(I)>	15.6
Completeness [%]	92.5	95.4
Redundancy	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	4 *	60 mM PIPES 200 mM CASO4 0.1 % 1,4 Dioxane 15% Glycerol 15% PEG MME 5000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PIPES	60 (mM)
2	1	reservoir		200 (mM)
3	1	reservoir	1,4-dioxane	0.1 (%(v/v))
4	1	reservoir	glycerol	15 (%(v/v))
5	1	reservoir	PEG5000 MME	15 (%(v/v))
6	1	drop	PHMB	0.1 (mM)
7	1	drop	Tris	10 (mM)