1CZ0
INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-20 |
| Detector | OTHER |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.866, 113.866, 89.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.21600 |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.180 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.067 | 0.178 |
| Total number of observations | 206866 * | |
| Number of reflections | 38078 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 96.9 | 97.8 |
| Redundancy | 5.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 4 * | Flick, K.E., (1997) Protein Sci., 6, 2677. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CITRATE | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | EDTA | ||
| 5 | 1 | 1 | PEG 3350 | ||
| 6 | 1 | 2 | PEG 3350 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | EDTA | 2 (mM) | |
| 4 | 1 | reservoir | citrate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG4000 | 21-27 (%) |
