1CY2
COMPLEX OF E.COLI DNA TOPOISOMERASE I WITH TPTPTP3'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-04-01 |
Detector | PRINCETON 2K |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.750, 78.040, 139.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 2.300 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.28400 |
RMSD bond length | 0.006 |
RMSD bond angle | 21.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.085 | 0.174 |
Total number of observations | 545417 * | |
Number of reflections | 31167 | |
Completeness [%] | 96.3 | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 4 * | and 14 degrees centigrade * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5-6 (mg/ml) | |
2 | 1 | drop | nucleotide | 1.45-20 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 2.2-2.3 (M) |