1CVW

Crystal structure of active site-inhibited human coagulation factor VIIA (DES-GLA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-10
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	94.850, 94.850, 114.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.280
R-factor	0.2049
Rwork	0.205
R-free	0.23880
RMSD bond length	0.006
RMSD bond angle	1.370
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.360
High resolution limit [Å]	2.280	2.280
Rmerge	0.067	0.294
Total number of observations	345306 *
Number of reflections	22596
<I/σ(I)>	22.8
Completeness [%]	92.3	72.8
Redundancy	4.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	used micro bridge *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)