1CVN

CONCANAVALIN A COMPLEXED TO TRIMANNOSIDE

Experimental procedure

Temperature [K]	298
Detector technology	DIFFRACTOMETER
Collection date	1995-07-13
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 1 21 1
Unit cell lengths	81.650, 66.680, 108.320
Unit cell angles	90.00, 97.79, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.205 *
Rwork	0.204
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cna
RMSD bond length	0.009
RMSD bond angle	26.540 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (2.1)
Refinement software	X-PLOR (2.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.085	0.222
Number of reflections	48025	5025 *
Completeness [%]	95.5	80
Redundancy	2.9	1.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9		pH 9.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	trimannoside	7 (mM)
3	1	reservoir	PEG6000	20 (%)