1CVJ
X-RAY CRYSTAL STRUCTURE OF THE POLY(A)-BINDING PROTEIN IN COMPLEX WITH POLYADENYLATE RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 1998-12-11 |
| Detector | OTHER |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.557, 176.797, 82.489 |
| Unit cell angles | 90.00, 117.67, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.30400 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.053 | 0.294 |
| Number of reflections | 61125 | |
| <I/σ(I)> | 19 | |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 24 * | a 1 : 1.2 protein : RNA ratio * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 24 * | a 1 : 1.2 protein : RNA ratio * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 24 * | a 1 : 1.2 protein : RNA ratio * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | TCEP | ||
| 2 | 1 | 1 | TRIS-HCL | ||
| 3 | 1 | 1 | KCL | ||
| 4 | 1 | 1 | (NH4)SO4 | ||
| 5 | 1 | 1 | GLYCEROL | ||
| 6 | 1 | 2 | (NH4)SO4 | ||
| 7 | 1 | 2 | GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
| 3 | 1 | drop | protein | 5.5 (mg/ml) | |
| 4 | 1 | drop | RNA |
