1CVC
REDESIGNING THE ZINC BINDING SITE OF HUMAN CARBONIC ANHYDRASE II: STRUCTURE OF A HIS2ASP-ZN2+ METAL COORDINATION POLYHEDRON
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.700, 41.700, 73.000 |
| Unit cell angles | 90.00, 104.60, 90.00 |
Refinement procedure
| Resolution | ? - 2.300 |
| R-factor | 0.17 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.600 |
| Refinement software | PROLSQ |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | Alexander, R. S., (1993) Biochemistry, 32. 1510. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CAII | 0.3 (mM) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 3 (mM) | ||
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | 3 (mM) | ||
| 8 | 1 | reservoir | octyl beta-glucoside | 1 (mM) | |
| 9 | 1 | reservoir | ammonium sulfate | 1.75-2.5 (M) |
