1CV8
STAPHOPAIN, CYSTEINE PROTEINASE FROM STAPHYLOCOCCUS AUREUS V8
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 61.139, 61.139, 195.063 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.183 |
| Rwork | 0.200 |
| R-free | 0.23900 |
| Structure solution method | MIR |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.800 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.062 | 0.070 |
| Number of reflections | 19765 | |
| <I/σ(I)> | 20.8 | 10 |
| Completeness [%] | 82.3 | 63 |
| Redundancy | 2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 5 M AMMONIUM ACETATE, 2MM DTT, 5MM E-64, 50 MM MES/TRIS BUFFER PH 6.0 5MM E-64 |
