1CV2
Hydrolytic haloalkane dehalogenase linb from sphingomonas paucimobilis UT26 AT 1.6 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-18 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 50.264, 71.669, 72.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.580 |
R-factor | 0.1491 |
R-free | 0.20400 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bn6 |
RMSD bond length | 0.009 |
RMSD bond angle | 2.300 * |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 1.600 |
High resolution limit [Å] | 1.580 * | 1.575 |
Rmerge | 0.065 | 0.146 |
Number of reflections | 34513 * | |
<I/σ(I)> | 18.31 | 5.98 |
Completeness [%] | 94.2 | 81.4 |
Redundancy | 4.84 | 2.79 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 278 | microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 18-20 (%(w/v)) | |
2 | 1 | reservoir | calcium acetate | 0.2 (M) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) |