1CUS

FUSARIUM SOLANI CUTINASE IS A LIPOLYTIC ENZYME WITH A CATALYTIC SERINE ACCESSIBLE TO SOLVENT

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	35.120, 67.300, 37.050
Unit cell angles	90.00, 93.90, 90.00

Refinement procedure

Resolution	6.000 - 1.250
R-factor	0.158
Rwork	0.158
RMSD bond length	0.009
RMSD bond angle	2.080
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.250 *
Rmerge	0.061 *
Total number of observations	114297 *
Number of reflections	39563 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *	used to seed, referred to J.Mol.Biol. 215.215-216 1990 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	HEPES	0.1 (M)
3	1	drop	PEG6000	15-20 (%(w/v))
4	1	reservoir	HEPES	0.1 (M)
5	1	reservoir	PEG6000	15-20 (%(w/v))