1CUP

METHIONINE CORE MUTANT OF T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Spacegroup name	P 32 2 1
Unit cell lengths	60.900, 60.900, 97.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.890
R-factor	0.16
Rwork	0.160
RMSD bond length	0.020
RMSD bond angle	2.730
Data reduction software	AUTOSTRAT
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.820
High resolution limit [Å]	1.700	1.690
Rmerge	0.042	0.230
Number of reflections	16754
<I/σ(I)>	10.7
Completeness [%]	93.0	28.8
Redundancy		1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7 *	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol