1CUN
CRYSTAL STRUCTURE OF REPEATS 16 AND 17 OF CHICKEN BRAIN ALPHA SPECTRIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 1998-05-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 126.341, 201.145, 94.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.630 - 2.000 |
| R-factor | 0.216 * |
| Rwork | 0.220 |
| R-free | 0.26200 * |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.770 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 2.000 |
| Rmerge | 0.055 | 0.170 |
| Total number of observations | 387128 * | |
| Number of reflections | 75300 * | |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 91.3 | 79.5 |
| Redundancy | 4.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.99 (M) | |
| 2 | 1 | reservoir | sodium potassium tartrate | 0.12 (M) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 4 | 1 | drop | protein | 5 (mg/ml) | |
| 5 | 1 | drop | HEPES | 10 (mM) |
