1CUN

CRYSTAL STRUCTURE OF REPEATS 16 AND 17 OF CHICKEN BRAIN ALPHA SPECTRIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	108
Detector technology	CCD
Collection date	1998-05-01
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	126.341, 201.145, 94.765
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.630 - 2.000
R-factor	0.216 *
Rwork	0.220
R-free	0.26200 *
RMSD bond length	0.007 *
RMSD bond angle	1.770 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.060
High resolution limit [Å]	1.950	2.000
Rmerge	0.055	0.170
Total number of observations	387128 *
Number of reflections	75300 *
<I/σ(I)>	9.3
Completeness [%]	91.3	79.5
Redundancy	4.8	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.99 (M)
2	1	reservoir	sodium potassium tartrate	0.12 (M)
3	1	reservoir	sodium citrate	0.1 (M)
4	1	drop	protein	5 (mg/ml)
5	1	drop	HEPES	10 (mM)