1CUF
CUTINASE, R156L MUTANT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.120, 67.360, 37.050 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.750 |
| R-factor | 0.144 * |
| Rwork | 0.145 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.550 |
| Data reduction software | MARXDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.750 * |
| Rmerge | 0.051 |
| Total number of observations | 68385 * |
| Number of reflections | 15907 |
| Completeness [%] | 91.0 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG6000 | 2-20 (%(w/v)) | |
| 2 | 1 | drop | HEPES | 100 (mM) | |
| 3 | 1 | drop | cutinase | 5-15 (mg/ml) |
