1CU6

T4 LYSOZYME MUTANT L91A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Spacegroup name	P 32 2 1
Unit cell lengths	60.900, 60.900, 97.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.100
R-factor	0.165
Rwork	0.174
RMSD bond length	0.021 *
RMSD bond angle	2.700
Data reduction software	DENZO
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.700
Rmerge	0.040 *
Number of reflections	21321
Completeness [%]	94.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7 *		Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol