1CTW

T4 LYSOZYME MUTANT I78A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-01-01
Detector	RIGAKU RAXIS
Spacegroup name	P 32 2 1
Unit cell lengths	61.130, 61.130, 97.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.172
Rwork	0.172
RMSD bond length	0.019
RMSD bond angle	2.600
Data reduction software	DENZO
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.100
High resolution limit [Å]	2.050	2.050
Rmerge	0.088	0.510
Number of reflections	11183
<I/σ(I)>	7.1
Completeness [%]	83.0 *
Redundancy	6.1	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7 *	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol