1CTJ

CRYSTAL STRUCTURE OF CYTOCHROME C6

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	293
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1993-05
Detector	MARRESEARCH
Wavelength(s)	0.80, 0.87
Spacegroup name	R 3
Unit cell lengths	40.430, 40.430, 40.430
Unit cell angles	80.25, 80.25, 80.25

Refinement procedure

Resolution	25.000 - 1.100
R-factor	0.1377
R-free	0.18830
Structure solution method	AB INITIO
RMSD bond length	0.013
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.200
High resolution limit [Å]	1.100	1.100
Rmerge	0.058	0.520
Number of reflections	32653
<I/σ(I)>	12.1	2.4
Completeness [%]	97.9	93.5
Redundancy	8.4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium citrate	0.2 (M)
2	1	drop	glycine	0.025 (M)
3	1	drop	cytochrome	1.8 (mM)
4	1	drop	tricine	0.375 (mM)
5	1	reservoir	sodium citrate	0.8 (M)
6	1	reservoir	glycine	0.1 (M)