1CT9
CRYSTAL STRUCTURE OF ASPARAGINE SYNTHETASE B FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-11 |
| Detector | OTHER |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.930, 127.100, 204.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.29700 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.400 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.331 |
| Total number of observations | 1085969 * | |
| Number of reflections | 200469 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 98.0 | 93.6 |
| Redundancy | 5.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | PEG 8000, HEPES, MAGNESIUM CHLORIDE, L-GLUTAMINE, ADENOSINE 5'-MONOPHOSPHATE, SODIUM CHLORIDE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | glutamine | 5 (mM) | |
| 4 | 1 | drop | AMP | 10 (mM) | |
| 5 | 1 | drop | PEG8000 | 14 (%(w/v)) | |
| 6 | 1 | drop | HEPES | 50 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 15 (%(w/v)) | |
| 8 | 1 | reservoir | HEPES | 50 (mM) |
