1CSU

REPLACEMENTS IN A CONSERVED LEUCINE CLUSTER IN THE HYDROPHOBIC HEME POCKET OF CYTOCHROME C

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	36.510, 36.510, 137.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.810
R-factor	0.196
RMSD bond length	0.019
RMSD bond angle	0.037
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.810 *
Total number of observations	7920 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (mg/ml)
2	1	drop	ammonium sulfate	75 (%sat)
3	1	drop	sodium phosphate	0.1 (M)
4	1	reservoir	ammonium sulfate	90 (%sat)