1CSN
BINARY COMPLEX OF CASEIN KINASE-1 WITH MGATP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Detector technology | AREA DETECTOR |
| Wavelength(s) | 1.0, 1.0012 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 78.960, 78.960, 120.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.300 - 2.000 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.460 |
| Data reduction software | MADNES |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | |
| High resolution limit [Å] | 2.000 * | 2.000 * |
| Rmerge | 0.080 | |
| Total number of observations | 161782 * | |
| Number of reflections | 24245 | |
| Completeness [%] | 93.9 * | 44.2 * |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 16 * | Carmel, G., (1994) J.Biol.Chem., 269, 7304. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.3 (mM) | |
| 10 | 1 | drop | sodium citrate | 50 (mM) | |
| 11 | 1 | reservoir | ammonium sulfate | 1.55 (mM) | |
| 12 | 1 | reservoir | sodium citrate | 50 (mM) | |
| 2 | 1 | drop | MOPS | 10 (mM) | |
| 3 | 1 | drop | EDTA | 0.1 (mM) | |
| 4 | 1 | drop | 2-mercaptoethanol | 0.1 (%) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | 200 (mM) | ||
| 7 | 1 | drop | ATP | 6 (mM) | |
| 8 | 1 | drop | 1.5 (mM) | ||
| 9 | 1 | drop | ammonium sulfate | 1.55 (mM) |
