1CSM

THE CRYSTAL STRUCTURE OF ALLOSTERIC CHORISMATE MUTASE AT 2.2 ANGSTROMS RESOLUTION

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-10-19
Detector	RIGAKU
Spacegroup name	P 61
Unit cell lengths	95.800, 95.800, 157.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.196
Rwork	0.196
R-free	0.31000
RMSD bond length	0.110
RMSD bond angle	1.527
Data reduction software	MSC
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.086
Total number of observations	83251 *
Number of reflections	38840
Completeness [%]	80.6
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			Xue, Y., (1994) J. Mol. Biol., 241, 273. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	55 (%(w/v)sat)
3	1	reservoir	Tris-HCl	50 (mM)
4	1	reservoir	DTT	2 (mM)
5	1	reservoir	EDTA	0.1 (mM)