1CSH

A very short hydrogen bond provides only moderate stabilization of an enzyme: inhibitor complex of citrate synthase

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	104.400, 78.500, 58.500
Unit cell angles	90.00, 78.90, 90.00

Refinement procedure

Resolution	25.000 - 1.650
R-factor	0.164
RMSD bond length	0.014
RMSD bond angle	2.700
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.650 *
Rmerge	0.039 *
Total number of observations	73408 *
Number of reflections	43148 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	0.2 (mM)
2	1	drop	citrate	0.5 (M)
3	1	drop	oxaloacetate	5 (mM)
4	1	drop	inhibitor	0.5 (mM)
5	1	reservoir	sodium citrate	0.9-1.1 (M)