1CS6
N-TERMINAL FRAGMENT OF AXONIN-1 FROM CHICKEN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-02-20 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.583, 45.540, 99.548 |
Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.25700 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | CNS (0.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.036 | 0.206 |
Number of reflections | 48051 | |
<I/σ(I)> | 12.8 | |
Completeness [%] | 95.1 | 83.3 |
Redundancy | 2.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 * | 17 * | PEG 10000, sodium formiate, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 25 (mM) | |
3 | 1 | drop | 125 (mM) | ||
4 | 1 | reservoir | PEG10000 | 15-17 (%(w/v)) | |
5 | 1 | reservoir | sodium formiate | 150 (mM) | |
6 | 1 | reservoir | HEPES | 100 (mM) |